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Cytochalasin b glucose uptake

Web(B) Glucose uptake was assayed by incubation of control, cytochalasin B-, and cytochalasin D-treated Jurkat cells in the presence of 2-deoxy-D [1-3H]glucose (5 μM) … WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release.

Effects of cytochalasin B on the uptake of ascorbic acid …

WebCytochalasin B is a cell-permeable fungal toxin which binds to the barbed end of actin filaments inhibiting both the association and dissociation of subunits. It causes the disruption of actin filaments and inhibition of actin polymerization. ... Effect of cytochalasin B on glucose uptake, utilization, oxidation and insulinotropic action in ... WebA novel phosphoinositolglycan-peptide (PIG-P) from the yeast Saccharomyces cerevisiae potently mimicks insulin action on glucose transport and metabolism in rat muscle and adipose tissue. The aim of the present study was to elucidate the cellular flyers cates https://ashleysauve.com

Analysis of Insulin-Stimulated Glucose Uptake in ... - Springer

WebMay 1, 1972 · A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-D-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at very low... WebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range. WebIn Fig. 4 (right side), we summarize the re- sults of 4 -6 independent experiments, comparing the insulin-dependent net change in cytochalasin B binding sites with the net change in GLUT4 glucose ... flyers carrier

Signalling pathways of an insulin-mimetic ... - Academia.edu

Category:Effect of cytochalasin b on glucose uptake, utilization, …

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Cytochalasin b glucose uptake

Signalling pathways of an insulin-mimetic ... - Academia.edu

WebCytochalasin B, CAS: 14930-96-2, is a cell-permeable fungal toxin used in actin polymerization studies and cytological research.. Cited in 16 publications ... Effect of cytochalasin B on glucose uptake, utilization, oxidation and insulinotropic action in tumoral insulin-producing cells. Malaisse, WJ., et al. 1987. Cell Biochem Funct. 5: 183-7. WebCytochalasin B (50µM) was added to determine assay background. Glucose Uptake-Glo™ Assay We have developed a luminescent glucose uptake assay that is based on the same principle as the aforementioned …

Cytochalasin b glucose uptake

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WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. … WebFeb 1, 2013 · RBCs from Atlantic salmon showed a linear relationship between glucose uptake and extracellular glucose level, but exhibited clear inhibition of glucose metabolism by cytochalasin B and phloretin, suggesting a component of facilitated glucose transport that is more elusive to detect.

WebMay 23, 2003 · Glucose levels dropped even in the presence of the transport inhibitor cytochalasin B, indicating rapid metabolism. Consistently, the addition of 2 … WebApr 14, 2016 · Glucose uptake for GlcP Se was measured one minute after initiation of transport with 30 μM C 14 -glucose in the presence of 2 mM …

WebNov 10, 2014 · To further confirm our findings in primary brown adipocyte cultures, glucose uptake in BAT was measured in vivo in β 1 /β 2 knockout (to exclude any effects on β 1 - or β 2-adrenoceptors) and wild-type FVB mice that express all three β-adrenoceptor subtypes (Fig. 4, A and B). Isoproterenol increased glucose uptake in BAT in vivo in FVB and ... WebCytochalasin B, which is often used as an inhibitor of actin-based motility systems, is also a very potent competitive inhibitor of Đ-glucose uptake into mammalian cells. When red blood cell ghosts (yes it's a real term, these are RBCs emptied of their cytoplasm) are incubated when 'H-cytochalasin and then irradiated with ultraviolet light, the

WebApr 23, 2015 · Moreover, cytochalasin B, a nonspecific glucose transport inhibitor, also strongly inhibited the uptake of 14 C-glucose, 14 C-2DG and 2DG-IR in neuronal and astrocytic cultures .

WebA mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy- d -glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at … greenish discharge while pregnantWeb2.2 Assaying Insulin-Stimulated Glucose Uptake. 1. Serum-free media: DMEM (with 4.5 g/L glucose, l-glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin … greenish duck crossword clueWebJul 1, 1974 · Cytochalasin B in this concentration suppresses multiplication rates in a nutrient medium consisting of 2 % proteose peptone, but multiplication is unaffected if … greenish eye color crosswordWeb2.2 Assaying Insulin-Stimulated Glucose Uptake 1. Serum-free media: DMEM (with 4.5 g/L glucose, l -glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin (BSA). 2. 2-Deoxyglucose (Sigma) stock: 100 m M (16.4 mg/mL in double-distilled water). Store at 2°–4°C up to 2 wk. 3. greenish drainageWebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ... greenish expectorantWebMay 10, 1972 · A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is … greenish discharge in early pregnancyWebEnter the email address you signed up with and we'll email you a reset link. flyers cell phone cases