WebTube 2: Prepare 10 μL volume of diluted DharmaFECT transfection reagent in serum-free medium. Depending on the cell line and cell density the DharmaFECT reagent amount can vary between 0.05-0.5 μL WebAt the same time, Lipofectamin, Dharmafect 1, Dharmafect 2, Dharmafect 3 and Dharmafect 4 were complexed with the pCMV-EGFP plasmid at the reagent: DNA ratio respectively of 4 µl:1.5 µg for Lipofectamin and 2.5 µl:1.5 µg for the 4 Dharmafect reagents. Complexes were prepared by mixing the appropriate amount of lipofection reagent …

RNA interference screening methods to identify proliferation ...

Web2. Dilute cells in antibiotic-free medium to a plating density of 2.5 x 105 cells/mL for transfection with DharmaFECT 1 or 1.0 x 105 cells/mL for transfection with DharmaFECT 4. Complete medium is ... WebBlock 4: DharmaFect 2: 6.25: 181.3: Block 5: DharmaFect 3: 6.25: 181.3: Block 6: DharmaFect 4: 6.25: 181.3: Block 7: RNAiMax: 3.75: 183.8: Open in a separate window. For siRNA: 1 μM siRNA is diluted in Opti-MEM (1:3) and 7 μl diluted siRNA is added to each corresponding well. Each siRNA is dispensed into 21 wells, therefore, 147 μl are needed. china express mantoloking road brick nj

Development of EphA2 siRNA-loaded lipid nanoparticles and …

Web• For final volumes of DharmaFECT Transfection Reagent that are different than those described in Table 4, please adjust the volumes of stock DharmaFECT Transfection Reagent and cell culture medium accordingly. • The Rehydration Solution may be stored under sterile conditions at room temperature for up to 2 hours prior to use. 3. WebNov 9, 2016 · Cells were transfected with 100 nM siRNA against SCD1 (SMARTpool reagent; Dharmacon, Chicago, IL, USA) or control siRNA (nontargeting siRNA; Dharmacon) using DharmaFECT 4 transfection reagent (Dharmacon), and incubated for 24 h in 0.2% FCS-containing DMEM. Following transfection, the cells were starved for 24 h, treated … WebTransfection Reagents Product Brochure - Thermo Scientific china express menu springfield il